Extraction of dna from bacteria

Extraction of dna from bacteria

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Each tube should contain about 5 ml of the E. colisolution or be half full, whichever is less. We report a quick and low-cost gDNA extraction protocol called EtNa that is efficient for bacteria and yeast over a broad range of concentrations. EtNa is based on a hot alkaline ethanol lysis. The solution can be immediately centrifuged to yield a crude gDNA extract suitable for PCR, or it can be directly applied to a silica column for purification. Molecular biology techniques such as PCR, mass spectrometry, and sequencing have been optimized in order to speed up the detection and analysis of microorganisms ( 1).

It has been extraction of dna from bacteria to the author(s) of this protocol.




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