Extraction of dna from onion cells
Thisprocedure is designed to extract DNA from onion in sufficientquantity to be seen and spooled. Clientele: 13 years and upType of resourceExperiment to do in class.Printable version of this pageProtocole - Extracting DNA from onions (PDF 4 Ko)ObjectivesThe students will extract deoxyribonucleic acid (DNA) from tissues of a living organism (the onion) using grocery products.Description of the activityDNA is the genetic material of all living organisms. Within this master molecule, all the information necessary to create and direct the chemical machinery of life is found.
Immediately incubate in ice water for 5 minutes5. Filter through 4 layers of cheese cloth. To 3 ml filtrate, add 5 ml ice cold iso-propanol along the sides of the test tube without shakingKeep this test tube undisturbed for 3-5 minutes in iceWhite strandDNA (deoxyribonucleic acid) can be extraction of dna from onion cells from materialby homogenizing, deproteinizing, and precipitating it fromsaid material.Procedure:The first step in DNA isolation is homogenization, whichinvolves breaking down and removing cell walls andmembranes. I prepared a medium consisting of 50.0 g ofsodium dodecyl sulfate (SDS) 8.77 g of sodium chloride(NaCl), 4.41 g of sodium citrate, 0.292 g ofethylenediamine tetraacetic acid (EDTA), and enoughdistilled water to make 1 liter of solution.
DNA and Litmus PaperWhen the DNA sample was tested with litmus paper, the paper did not change colour at all.(see image 11)The DNA sample was supposed to change the litmus paper red because DNA is an acid (made up of nucleic acids - Deoxyribonucleic acid) and acids are supposed to turn blue litmus paper red.Sources of error: not enough DNA on the rod to rub on the litmus paper - we had difficulty getting even a single DNA strand onto the rod. writing paper online india Also, the extraction solution was still on the rod (and the extraction solution is basic) so this could have been the reason why the litmus p.